28.1.177 UncompInhib

Function

y=\frac{V_{\text{max}}x}{(1+\frac{I_\text{c}}{K_\text{ia}})(\frac{K_\text{m}}{1+\frac{I_\text{c}}{K_\text{ia}}}+x)}

Brief Description

Uncompetitive inhibition model for single substrate and single inhibitor.

Sample Curve

UncompInhib.png

Parameters

Number: 4

Names: Vmax, Km, Kia, Ic

Meanings: Vmax = Maximum enzyme velocity, Km = Michaelis-Menten constant, Ki = Inhibitor constant, Ic = Inhibitor concentration

Lower Bounds: Vmax > 0, Km > 0, Kia >0

Upper Bounds: none

Note that this function is usually used in global fit, Vmax, Km and Kia should be shared, and Ic be fixed for each dataset. The initial value of Kia can be the mean of Ic.

Derived Parameters

Script Access

nlf_UncompInhib(x,Vmax,Km,Kia,Ic)

Function File

fitfunc\UncompInhib.fdf

Category

Enzyme Kinetics