File Exchange > Stats >    Volcano Plot

How to install and run
Author:
OriginLab Technical Support
Date Added:
3/11/2016
Last Update:
6/17/2026
Downloads (90 Days):
603
Total Ratings:
32
File Size:
53 KB
Average Rating:
File Name:
VolcanoPlot.opx
File Version:
1.23
Minimum Versions:
2018 (9.5)
License:
Free
Type:
App
Summary:

Create volcano plot for fold change and p-value data.

Screen Shot and Video:
Description:

PURPOSE
This tool can be used to create scatter plot of p-value versus fold change for microarray data. The graph is composed of six regions. The plot style for each region can be individually customized.

INSTALLATION
Download the file VolcanoPlot.opx, and then drag-and-drop onto the Origin workspace. An icon will appear in the Apps Gallery window.

OPERATION

  1. Click the Volcano Plot icon in the Apps Gallery window to open the dialog.
  2. Choose XY data from a worksheet: fold change for X and p-value for Y. If gene names or probe set IDs are available in the worksheet, choose them as Label.
  3. If X data is linear, check Log2 Transform for X check box to convert to log 2 scale.
  4. In Cutoff Values group, specify cutoff values for P-value and Fold Change. P-value must be between 0 and 1. Fold change must be larger than 1. Both of these two cutoff values are linear scale.
  5. Check Show Reference Lines option to add a horizontal line for the cutoff P-value and two vertical lines for the cutoff fold change in the plot.

NOTES

  1. To customize symbol color or size for each region of the plot, double click on the region to open Plot Details dialog, and change settings in the Symbol tab.
  2. To customize reference line style, right click on the line, then  choose Properties from the context menu. In the Object Properties dialog, change settings in the Line tab.

Updates:

v1.23 6/2/2026 Updated localization.
v1.22 11/13/2025 Fixed a bug.
v1.21 6/17/2025 Supported group and multiple P-values.
v1.1 11/13/2017 Fixed treenode bug in Origin 2018.

Reviews and Comments:
11/26/202517333529520good

11/22/20252335650566good

11/22/20252335650566good

11/21/20252335650566good

11/21/20252335650566good

07/09/2025Jiasx19790916great

05/06/202576324982good

04/27/2025Lxx1561

04/27/2025Lxx1561

03/23/2025Panrry-001good

02/27/2025POLO1212121good

06/17/2024amanda919good

03/21/2024u308416hI wonder what is the base for the logFC? Is it 10 or 2?

03/21/2024u308416hI wonder what is the base for the logFC? Is it 10 or 2?

07/09/2023dandan.panwell good

02/13/2023OriginLabHi, giorgiocorsi

Could you let us know which parameters you feel are not clear yet?

OriginLab Technical Support

02/10/2023giorgiocorsiWorks fine, but some settings/parameters should be better explained

07/19/2022CSK002411111

03/10/2022yukiOriginlabHi ORIGINLABTEST2, could you please tell us what went wrong when you were using this app? We can help to fix the problem.
OriginLab Technical Support

03/10/2022ORIGINLABTEST2does not work

03/07/2022OriginLabHi dhmast_pnnl,
I am afraid we do not reproduce your question in Origin2021. If you do not mind, please send your project to us, then we can reproduce this issue.
https://www.originlab.com/restricted/support/newticket.aspx?c=3
Thanks
OriginLab

03/04/2022dhmast_pnnlNot working in OriginPro 2021. I Press okay, but the plot doesn't load in a new window. nothing happens

11/24/2021396936023good

10/24/2021OriginLabHi xiasang0506, please make sure that you are using this app on Origin 2016 or later versions. If you still have a problem, please contact us via https://www.originlab.com/restricted/support/newticket.aspx?c=3
OriginLab Technical Support

10/23/2021xiasang0506it does not work! worst!

06/28/2021its-helpdesk 

03/16/2021yangyang1231

09/03/2020OriginLabHi Dirk Hubmacher,

Would you please contact us via:tech@originlab.com to tell us the requirement about adding labels in details?

Thanks,
Shirley
OriginLab

09/02/2020Dirk HubmacherHow can I add the labels on the plot?

07/28/2020Hongxia Zhang 

09/19/2019Bolin2019This software is very useful and convenient.

09/18/2016rexlee I have student version of origin pro 2016. I cannot open this file. Can you please help me with this?