Description:
This graph presents an example of a multiple comparison plot. The bars and the pairwise comparison annotations were generated automatically by a Welch's ANOVA, which produced paired comparison plots for all five groups. The asterisk brackets indicate the significance of each comparison of a group against the control.
Background: mPTP opening was measured in five groups of cardiomyocytes: normal control, OGD/R model, OGD/R + DST-3 (1 μM), OGD/R + DST-3 (2 μM), and OGD/R + CTS (1 μM). Because equal variances could not be assumed, the overall difference among the five groups was assessed by Welch's ANOVA, followed by multiple comparisons in which each group was compared with the OGD/R model as the control level, using the Games–Howell test. The results showed that OGD/R markedly increased mPTP opening relative to the normal control, whereas DST-3 attenuated this effect and CTS also reduced mPTP opening relative to the OGD/R model (all comparisons ***, p ≤ 0.001).