Integrating Peaks

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Use the Peak Analyzer to integrate peaks and find their areas.

Minimum Origin Version Required: Origin 8.0 SR6

What you will learn

  • How to pick an existing dataset as baseline
  • How to subtract a baseline from the spectrum data
  • How to calculate peak areas with the Peak Analyzer


  1. Start a new workbook and import the file \Samples\Spectroscopy\Peaks with Base.DAT.
  2. Highlight the second column.
    Integrating Peaks 01.png
  3. In the main menu, click Analysis, then point to Peaks and Baseline, and then click Peak Analyzer.
  4. In the first page (the Goal page) of the Peak Analyzer, select Integrate Peaks in the Goal group. Click Next to go to the Baseline Mode page.
    Integrating Peaks 02.png
  5. On the Baseline Mode page, select Use Existing Dataset from the Baseline Mode dropdown list. Click the triangular button to the right of the Dataset dropdown menu, and then select [PeakswithBase]"Peaks with Base"!C"Base" on the fly-out menu. Click Next to go to the Baseline Treatment page of the Peak Analyzer.
    Integrating Peaks 03.png
  6. Select the Auto Subtract Baseline check box. Click the Integrate Peaks page icon in the upper panel (or click Next twice to go to the Integrate Peaks page). In the preview graph, you will see two numbered yellow rectangles representing two peaks found with the default settings.
    Integrating Peaks 04.png
  7. For Integration Window Width, select Adjust on Preview Graph. On the graph, click inside the rectangle marked with 1. A pair of "handles" appears on either side of the yellow integration window. Drag these handles to adjust the range for which integration will be performed. Click inside the rectangle marked with 2 and adjust the integration range for the second peak.
    Integrating Peaks 05.png
  8. In the Integrate Peaks page of the Peak Analyzer, make sure all the desired quantities to compute have been selected in the Quantities group. For example, if you want to calculate the peak centroid for each peak, select the Peak Centroid check box. If you don't want to output the percent areas, clear the Percent Area check box. When you are done, click Finish to perform the analysis.
    Integrating Peaks 06.png
    The result is in a worksheet named Integration_Result1.
    Integrate peak result.png
    If the Area check box was selected in the Quantities group, peak area data appears in the Area column of the result worksheet.